dat sc 32258 goat  (R&D Systems)

Journal: The Journal of nutrition

Article Title: Severe postnatal iron deficiency alters emotional behavior and dopamine levels in the prefrontal cortex of young male rats.

doi: 10.3945/jn.111.145946

Figure Lengend Snippet: FIGURE 2 Microdialysis measurements of extracellular resting and stimulated dopamine (A) and Western-blot analysis of DAT and D2R (B) in prefrontal cortex of rats fed a control iron-deficient diet for 4 wk. Data are mean 6 SEM, n = 4. *Different from control, P , 0.05 (Wilcoxon’s Rank Sum test). Western blot shows 3 representative samples with similar results observed for 7–8 different rats in each group. D2R, dopamine D2 receptor; DAT, dopamine transporter.

Article Snippet: The membrane was blocked in 5 mmol/L Tris base, 150 mmol/L NaCl, pH 7.4 containing 5% nonfat dry milk, and incubated in goat anti-human dopamine transporter (DAT) antibody (Santa Cruz; 1:100; Supplemental Fig. 1) or mouse anti-human dopamine D2 receptor (D2R) antibody (Santa Cruz, 1:100) in the same buffer containing 0.1% Tween 20 and 2% nonfat dry milk.

Techniques: Western Blot, Control